Histochemical and surface microstructural analyses of floral cuticles provide evidence for differential behaviors in scent volatiles emission in two tuberoses (Agave amica (Medik.) Theide and Govaerts) cultivars from Asparagaceae

Two popular cultivars of tuberose (Agave amica (Medik.) Theide and Govaerts syn. Polianthes tuberosa L.) from the family Asparagaceae, widely cultivated for floricultural purposes are ,'Calcutta Single' (CS) 'Calcutta Double' (CD). It was evidenced that CS flowers emit intense scent volatiles as compared to CD flowers, while retained higher contents internal pool than flowers. Such contrasting observations between two suggest role possible barriers such cuticles prevent emission volatiles. The cuticular structure both studied using different histochemical approaches, including light epi-fluorescence microscopies. Scanning electron microscopy (SEM) used understand deposition in cultivars. Our confirmed relatively thickness CS. SEM results showed accumulation wax crystalloids flower formation nanoridges over surfaces tepals. In addition, lipid droplets also noticed on tepal peels showing a denser CD. presence enhances volatiles, thick possibly deters accumulated an amounts. stomatal behavior indicates proportional relationship emission; low corresponds floral vice versa observed respectively.